PCR Enzyme
  • AP131-11.JPG
AP131-11.JPG

TransTaq? DNA Polymerase High Fidelity (HiFi) (含 2.5 mM dNTPs)


產品介紹

TransTaq? DNA Polymerase High Fidelity (TransTaq? HiFi DNA Polymerase)是以TransTaq?-T DNA Polymerase為主的復合酶TransTaq? HiFi DNA Polymerase具有5'→3' DNA聚合酶活性,5'→3'外切酶活性和3'→5'外切酶活性。不同模板使用不同Buffer實現高效擴增。TransTaqHiFi Buffer I 適用于基因組DNA的擴增。TransTaqHiFi   Buffer II  適用于 λDNA,cDNAPlasmid DNA的擴增。
保真性是EasyTaq? DNA Polymerase 18倍。
? 延伸速度為1-2 kb/min
擴增產物3' 端帶"A"堿基,可直接克隆于pEASY?-T載體中。
? 基因組DNA片段的擴增 (≤15 kb)。

產品組成

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References

Li P, Du J, Goodier J L, et al. Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent 
ORF1p depletion[J]. Nucleic acids research, Nucleic Acids Res. 2017, 45(8):4619-4631.

Che L H, Zhang SQ, Li Y, et al. Genome-wide survey of nuclear protein-coding markers for beetle phylogenetics and their application in resolving both deep 
and shallow-level divergences[J]. Molecular Ecology Resources, 2017, 17(6):1342.

You CX , Zhao Q , Wang XF , et al. A dsRNA-binding protein MdDRB1 associated with miRNA biogenesis modifies adventitious rooting and tree architecture 
in apple[J]. Plant Biotechnology Journal, 2014, 12(2):183-192.

Gu P , Yang F , Kang J , et al. One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in 
Escherichia coli[J]. Microbial Cell Factories, 2012, 11(1):30.

Wang G H , Sun B F , Xiong T L , et al. Bacteriophage WO Can Mediate Horizontal Gene Transfer in Endosymbiotic Wolbachia Genomes[J]. Frontiers in 
Microbiology, 2016, 7:1867.


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