PCR Enzyme
  • AP131-11.JPG

TransTaq? DNA Polymerase High Fidelity (HiFi) (含 2.5 mM dNTPs)


TransTaq? DNA Polymerase High Fidelity (TransTaq? HiFi DNA Polymerase)是以TransTaq?-T DNA Polymerase為主的復合酶TransTaq? HiFi DNA Polymerase具有5'→3' DNA聚合酶活性,5'→3'外切酶活性和3'→5'外切酶活性。不同模板使用不同Buffer實現高效擴增。TransTaqHiFi Buffer I 適用于基因組DNA的擴增。TransTaqHiFi   Buffer II  適用于 λDNA,cDNAPlasmid DNA的擴增。
保真性是EasyTaq? DNA Polymerase 18倍。
? 延伸速度為1-2 kb/min
擴增產物3' 端帶"A"堿基,可直接克隆于pEASY?-T載體中。
? 基因組DNA片段的擴增 (≤15 kb)。




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