PCR Enzyme
  • AP141-01.JPG
AP141-01.JPG

TransStart? Taq DNA Polymerase


產品介紹

TransStart? Taq DNA Polymerase是利用“TransStart熱啟動技術研發的新型熱啟動酶,是利用兩種DNA結合蛋白分別與引物和模板高效結合,一種結合蛋白和引物結合,阻止了低溫下引物形成二聚體;另一種結合蛋白和模板結合,阻止了低溫下DNA聚合酶與DNA模板結合。隨著變性步驟的進行,兩種蛋白失活,釋放的引物與模板參與擴增反應,增強PCR擴增效率和擴增特異性。擴增產物3'端帶“A”堿基,可直接克隆于pEASY?-T系列載體中。

? 保真性是EasyTaq? DNA Polymerase 18倍。

? 特異性優于抗體封閉和化學封閉熱啟動DNA 聚合酶。
? 室溫配制反應,減少非特異擴增和引物二聚體。
? 不使用Taq抗體,減少了潛在的來源于哺乳動物DNA污染的風險。
? 不同于化學修飾的Taq,無需加熱步驟,避免損傷DNA模板和降低DNA Polymerase活性。

? 基因組DNA 片段的擴增 (≤15 kb)。

產品組成

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References

Li S , Zhou L , Yao Y , et al. A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified
luminescent proximity homogeneous assays[J]. Chemical Communications, 2016, 53.

Yu C Y , Yin B C , Ye B C . A universal real-time PCR assay for rapid quantification of microRNAs via the enhancement of base-stacking hybridization[J].
Chemical Communications, 2013, 49(74):8247.

Bai C , Liu X , Fisher M C , et al. Global and endemic Asian lineages of the emerging pathogenic fungus Batrachochytrium dendrobatidis widely infect
amphibians in China[J]. Diversity & Distributions, 2012, 18(3):307-318.

Lin X , Li N , Kudo H , et al. Genes Sufficient for Synthesizing Peptidoglycan are Retained in Gymnosperm Genomes, and MurE from Larix gmelinii can Rescue
the Albino Phenotype of Arabidopsis MurE Mutation[J]. Plant and Cell Physiology, 2017, 58(3):587-597.

Song Y , Zhao G , Zhang X , et al. The crosstalk between Target of Rapamycin (TOR) and Jasmonic Acid (JA) signaling existing in Arabidopsis and cotton[J].
Scientific Reports, 2017, 7:45830.

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