點突變
  • FM111-01.JPG
FM111-01.JPG

Fast Mutagenesis System


產品介紹

計原理:

(1) 引物與模板鏈退火,2xTransStart? FastPfu Fly PCR SuperMix合成突變鏈;(2) DMT酶消化體外降解非突變型質粒模板(甲基化質粒模板)和DMT感受態細胞體內降解非突變型質粒模板(甲基化質粒模板),從而有效地篩選突變克隆。


產品組成

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References

Wang J, Zhou L, Shi H, et al. A single transcription factor promotes both yield and immunity in rice[J].Science, 2018, 7, 361(6406):1026-1028.
Li W , Zhu Z , Chern M , et al. A Natural Allele of a Transcription Factor in Rice Confers Broad-Spectrum Blast Resistance[J]. Cell, 2017, 170(1):114-126.e15.
Zhou X, Wang B, Pan Q, et al. Whole-genome sequencing of the snub-nosed monkey provides insights into folivory and evolutionary history[J]. Nature Genetics, 2014, 46(12):1303-1310.
Zhang H , Lin X , Han Z , et al. Crystal structure of PXY-TDIF complex reveals a conserved recognition mechanism among CLE peptide-receptor pairs[J]. Cell Research, 2016, 26(5):543-55.
Song N , Liu Z S , Xue W , et al. NLRP3 Phosphorylation Is an Essential Priming Event for Inflammasome Activation[J]. Molecular Cell, 2017, 68(1):185-197.

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