點突變
  • FM201-01.JPG
FM201-01.JPG

Fast MultiSite Mutagenesis System


產品介紹

本產品通過在重疊區域引入突變位點的方法,利用TransStart? FastPfu Fly PCR SuperMix 擴增,制備包含突變的PCR片段;利用特殊的重組酶和同源重組原理,將不多于6個片段無縫拼接,從而實現多點突變

產品組成

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References

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Du G , Gu Y , Hao C , et al. The cellular distribution of Na + /H +, exchanger regulatory factor 1 is determined by the PDZ-I domain and regulates the malignant progression of breast cancer[J]. Oncotarget, 2016, 7(20):29440-29453.
Chen Y , Luo Q , Zhou W , et al. Improving the catalytic efficiency ofBacillus pumilusCotA-laccase by site-directed mutagenesis[J]. Applied Microbiology and Biotechnology, 2017, 101(5):1935-1944.
Zeng F M , Wang X N , Shi H S , et al. Fascin phosphorylation sites combine to regulate esophageal squamous cancer cell behavior[J]. Amino Acids, 2017, 49(5):943-955.
Lu Y X , Liu X X , Liu W B , et al. Identification and characterization of Two types of Amino Acid-regulated acetyltransferases in Actinobacteria[J]. Bioscience Reports, 2017, 37(4):BSR20170157.

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