pEASY 系列克隆載體
  • CT501-01.JPG
CT501-01.JPG

pEASY?-T5 Zero Cloning Kit


產品介紹

該載體通過自殺基因表達與否篩選陽性重組子。當載體與片段連接成功時,自殺基因無法正確表達,包含重組子的細胞可以正常生長;當載體與片段連接不成功時,自殺基因正確表達,包含載體的細胞無法生長,即“Zero”背景。適用于TA克隆。


產品組成

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References

Xia B , Han D , Lu X , et al. Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale[J]. Nature Methods, 2015, 12(11):1047-50.
Gao Y , Chen J , Li K , et al. Replacement of Oct4 by Tet1 during iPSC Induction Reveals an Important Role of DNA Methylation and Hydroxymethylation in Reprogramming[J]. Cell Stem Cell, 2013, 12(4):453-469.
Le R , Kou Z , Jiang Y , et al. Enhanced Telomere Rejuvenation in Pluripotent Cells Reprogrammed via Nuclear Transfer Relative to Induced Pluripotent Stem Cells[J]. Cell Stem Cell, 2014, 14(1):27-39.
Gao S , Zheng C , Chang G , et al. Unique features of mutations revealed by sequentially reprogrammed induced pluripotent stem cells[J]. Nature Communications, 2015, 6:6318.
High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells[J]. Cell Research, 2014, 24(3):293-306.

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