pEASY 系列克隆載體
  • CB501-01.JPG
CB501-01.JPG

pEASY?-Blunt Zero Cloning Kit


產品介紹

該載體通過自殺基因表達與否篩選陽性重組子。當載體與片段連接成功時,自殺基因無法正確表達,包含重組子的細胞可以正常生長;當載體與片段連接不成功時,自殺基因正確表達,包含載體的細胞無法生長,即“Zero”背景。適用于平端克隆。


References

Gao F , Zhao J , Wang X , et al. NdhV Is a Subunit of NADPH Dehydrogenase Essential for Cyclic Electron Transport in Synechocystis sp. Strain PCC 6803[J]. Plant Physiology, 2016, 170(2):752.
He W , Huang T , Tang Y , et al. Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB[J]. Scientific Reports, 2015, 5(1):12368.
Hongyan W , Xiaoli T , Honglei W , et al. Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress[J]. Frontiers in Plant Science, 2015, 29(6):792.
Gao J , Wang G , Ma S , et al. CRISPR/Cas9-mediated targeted mutagenesis inNicotiana tabacum[J]. Plant Molecular Biology, 2015, 87(1-2):99-110.
Zhang X , He L , Zhang F , et al. The Different Potential of Sponge Bacterial Symbionts in N2 Release Indicated by the Phylogenetic Diversity and Abundance Analyses of Denitrification Genes, nirK and nosZ[J]. PLOS ONE, 2013, 10, 8(6):e65142.

FAQ