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蛋白檢測
  • DQ101-01.JPG
DQ101-01.JPG

Easy Protein Quantitative Kit (Bradford)


產品介紹

Bradford 蛋白定量方法,原理是在酸性條件下,考馬斯亮藍G-250 和蛋白質結合產生特殊的藍色,最大吸收波長在595 nm 處,顏色反應可5-10 分鐘內完成,20 分鐘內最穩定,測定效果最好。因此可通過比色分析在幾分鐘內快速測定蛋白質含量。與其它蛋白定量方法(如Lowry 法)相比,該方法測定蛋白濃度不受樣品中化學物質的影響。

產品組成

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References

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Zhang K , Zhao W J , Leng X Y , et al. The importance of the last strand at the C-terminus in βB2-crystallin stability and assembly[J]. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2014, 1842(1):44-55.

Yu-Rong L , Wen-Xiu M , Yi-Zhou C , et al. A Highly-Conserved Single-Stranded DNA-Binding Protein in Xanthomonas Functions as a Harpin-Like Protein to Trigger Plant Immunity[J]. PLoS ONE, 2013, 8(2):e56240.

Fang-Cheng B , Quan-Fang Z , Zhe L , et al. A Conserved Cysteine Motif Is Critical for Rice Ceramide Kinase Activity and Function[J]. PLoS ONE, 2011, 6(3):e18079.

Che Y Z , Li Y R , Zou H S , et al. A novel antimicrobial protein for plant protection consisting of a Xanthomonas oryzae harpin and active domains of cecropin A and melittin.[M]. Microbial Biotechnology. 2011, 4(6):777-93.