蛋白檢測
  • DQ111-01.JPG
DQ111-01.JPG

Easy II Protein Quantitative Kit (BCA)


產品介紹

BCA蛋白定量方法是目前最常用的兩種蛋白定量方法之一,其原理是在堿性條件下,蛋白質中的肽鍵能將Cu2+還原成Cu1+(雙縮脲反應),且Cu2+的還原量與總蛋白量成正比,生成的Cu1+能夠與BCA形成穩定的紫色化合物。與Bradford方法相比,BCA蛋白定量方法更加客觀,普通的肽鍵即可發生顏色反應,亦可兼容高濃度的SDS(5%),Triton X-100(5%),NP 40(5%),CHAPS(5%),Tween 20,60,80(5%),其弊端是易受某些還原劑(如二硫蘇糖醇和β-硫基乙醇等)、螯合劑(EDTA,EGTA)和高濃度緩沖液的影響,但這些干擾作用可以通過對蛋白質樣品的稀釋進行消除。  

產品組成

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References

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