蛋白純化
  • DP101-01.JPG
DP101-01.JPG

ProteinIso? Ni-NTA Resin


產品介紹

ProteinIso?  Ni-NTA Resin是螯合金屬Ni2+而形成的一種親和層析介質。NTA能夠通過四個位點牢固的螯合Ni2+從而減少純化過程中Ni2+泄露到蛋白樣品中。本產品對His標簽蛋白具有特異吸附能力,從而使His標簽蛋白結合在Ni-NTA純化介質上,未結合的蛋白被洗滌下去,結合在介質上的蛋白經過一定濃度的咪唑或低pH緩沖液溫和洗脫下來,從而得到高純度的目的蛋白。本產品具有吸附容量大、選擇性好,通透性強,易于再生的優點。它可用于在非變性或變性條件下純化任何表達系統表達的His標簽重組蛋白。  

References

Molecular characterization of LvAV in response to white spot syndrome virus infection in the Pacific white shrimp (Litopenaeus vannamei)[J]. Developmental & Comparative Immunology, 2015, 51(1):48-55.

Cao H, Yin X, Cao Y, et al. FCHSD1 and FCHSD2 Are Expressed in Hair Cell Stereocilia and Cuticular Plate and Regulate Actin Polymerization In Vitro[J]. PLOS ONE, 2013, 8(2):e56516.

Yang X Q, Wang W, Tan X L, et al. Comparative Analysis of Recombinant Cytochrome P450 CYP9A61 from Cydia pomonella Expressed in Escherichia coli and Pichia pastoris[J]. Journal of Agricultural and Food Chemistry, 2017, 65(11):2337-2344.

Cheng S , Li C , Wang Y , et al. Characterization and expression analysis of a thioredoxin-like protein gene in the sea cucumber Apostichopus japonicus[J]. Fish & Shellfish Immunology, 2016, 58:165-173.

Liu Y, Hou F, Wang X, et al. Recombinant expression and characterization of a serine protease inhibitor (Lvserpin7) from the Pacific white shrimp, Litopenaeus vannamei[J].2015 Feb;42(2):256-63.

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