RT-PCR
  • AH341-01.JPG
AH341-01.JPG

TransScript? II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)


產品介紹

本產品含有反轉錄反應所需的全部試劑(TransScript? II RT, RNase Inhibitor, Anchored Oligo(dT)20 Primer, Random Primer(N9), dNTPs, Buffer),濃度為。反應時,只需加入gDNA Remover、模板RNA和水,在42℃-55℃條件下即可高效地合成第一鏈cDNA,同時去除RNA模板中殘留的基因組DNA。另配有TransScript? II All-in-One No-RT Control SuperMix for qPCR,用于配制無反轉錄酶的對照,判斷qPCR模板是否來自cDNA。該產品操作簡便,降低操作過程中的污染機率。cDNA只適用于qPCR,不適用于常規PCR

產品組成

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使用前請將各組分點甩離心

References

Cui X . Exploring the formation and recognition of an important G-quadruplex in a HIF1α promoter and its transcriptional inhibition by a benzo[c]phenanthridine derivative.[J]. Journal of the American Chemical Society, 2014, 136(6):2583.

Gu Q , Chen Z , Yu X , et al. Melatonin confers plant tolerance against cadmium stress via the decrease of cadmium accumulation and reestablishment of microRNA-mediated redox homeostasis[J]. Plant Science, 2017, 261:28-37.

Huang X , Huang B , Chen J , et al. Cellular responses of the dinoflagellate\r, Prorocentrum donghaiense\r, Lu to phosphate limitation and chronological ageing[J]. Journal of Plankton Research, 2016, 38(1):83-93.

Guo J , Zu G , Zhou T , et al. Clinicopathological significance of orphan nuclear receptor Nurr1 expression in gastric cancer[J]. Clinical and Translational Oncology, 2015, 17(10):788-794.

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