PCR SuperMix
  • AS111-11.JPG
AS111-11.JPG

EasyTaq? PCR SuperMix (+dye )


產品介紹

本產品包含EasyTaq? DNA聚合酶、dNTPs和優化的反應緩沖液,濃度為2×。DNA擴增時,只需加入模板、引物和水,使SuperMix溶液的濃度為即可進行反應。擴增產物3' 端帶“A”堿基,EasyTaq? PCR SuperMix (-dye) 擴增產物可直接克隆于pEASY?-T系列載體中;EasyTaq? PCR SuperMix (+dye) 擴增產物可直接點樣電泳,如用于克隆,需純化去掉染料。PCR產物不適用于聚丙烯酰胺凝膠電泳
? 減少PCR擴增操作時間。
? 避免因多步操作帶來的污染。
? 基因組DNA片段的擴增 (≤4 kb)。

References

Han D, Liu J, Chen C, et al. Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells[J]. Nature. 2019 Feb;566(7743):270-274. 

Yang Y , Liu B , Xu J , et al. Derivation of Pluripotent Stem Cells with In?Vivo Embryonic and Extraembryonic Potency[J]. Cell, 2017, 169(2):243-257.e25.

Fu Q , Xu M , Chen X , et al. CEP78 is mutated in a distinct type of Usher syndrome[J]. Journal of Medical Genetics, 2017, 54(3):190-195.

Li Y , Zhang Q , Yin X , et al. Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules[J]. CELL RESEARCH, 2010, 21(1):196-204.

Yu C , Liu Y , Miao Z , et al. Retinoic acid enhances the generation of hematopoietic progenitors from human embryonic stem cell-derived hemato-vascular precursors[J]. Blood, 2010, 116(23):4786-4794.

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