PCR SuperMix
  • AS131-01.JPG
AS131-01.JPG

TransTaqHigh Fidelity (HiFi) PCR SuperMix I (-dye)


產品介紹

本產品含熱啟動的TransTaq? HiFi DNA PolymerasedNTPs和優化的反應緩沖液,濃度為2×。DNA增時,只需加入模板、引物和水,使SuperMix溶液的濃度為即可進行反應。擴增產物3'端帶“A”堿基,可直接克隆于pEASY?-T載體中。TransTaq HiFi PCR SuperMix I 適用于基因組DNA的擴增 (≤ 15 kb)。
? 減少PCR擴增操作時間。
? 避免因多步操作帶來的污染。
? 保真性是Easy
Taq? DNA Polymerase18倍。

References

Xu R , Qin R , Li H , et al. Generation of targeted mutant rice using a CRISPR-Cpf1 system[J]. Plant Biotechnology Journal, 2017 Jun, 15(6):713-717. 

Che L H , Zhang S Q , Li Y , et al. Genome-wide survey of nuclear protein-coding markers for beetle phylogenetics and their application in resolving both deep and shallow-level divergences[J]. Molecular Ecology Resources, 2017, 17(6):1342-1358.

Liu T , Sun L , Jiang B , et al. Lineage conversion of mouse fibroblasts to pancreatic α-cells[J]. Experimental & Molecular Medicine, 2017, 49(6):e350.

Hao J , Zhang C , Liang T , et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner[J]. Cellular and Molecular Immunology, 2014, 11(2):206-214.

Feng Y J , Liu Q F , Chen M Y , et al. Parallel tagged amplicon sequencing of relatively long PCR products using the Illumina HiSeq platform and transcriptome assembly[J]. Molecular Ecology Resources, 2016, 16(1):91-102.

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