細胞檢測
  • FC101-01.JPG
FC101-01.JPG

TransDetect? Cell  Counting Kit (CCK)


產品介紹

TransDetect? Cell Counting Kit,是一種基于水溶性四唑鹽的細胞增殖和細胞毒性檢測試劑盒。它在電子耦合試劑1-Methoxy PMS存在的情況下,可以被線粒體內的脫氫酶還原為可溶性的橙黃色甲臜(formazan)。甲臜的數量與活細胞的數量成正比。細胞增殖越快、細胞毒性越小、細胞數量越多,則顏色越深,顏色的深淺與細胞數量呈現良好的線性關系。該產品細胞毒性小,對后續實驗沒有影響,與MTTXTT,MTSWST-1相比,法檢測靈敏度更高,線性范圍更寬,適用于藥物篩選,細胞增殖測定,細胞毒性測定和腫瘤藥敏實驗。   

產品組成

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References

Li P, Du J, Goodier JL, et al. Aicardi-Goutières syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion.Nucleic Acids Res. 2017,45(8):4619-4631. 

Gu J , Wang D , Zhang J , et al. GFRα2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer[J]. Cancer Letters, 2016, 380(2):434-441.

Li J , Zhuang C , Liu Y , et al. Synthetic tetracycline-controllable shRNA targeting long non-coding RNA HOXD-AS1 inhibits the progression of bladder cancer[J]. Journal of Experimental & Clinical Cancer Research, 2016, 35(1):99.

Zhigang Zhu,a Jianliang Zhou,  Jingli Ding, et al. A novel approach to prepare a tissue engineering decellularized valve scaffold with poly(ethylene glycol)–poly(ε-caprolactone)[J]. RSC Adv. 2016, 6(17):14427-14438.

Min Q H , Wang X Z , Zhang J , et al. Exosomes derived from imatinib-resistant chronic myeloid leukemia cells mediate a horizontal transfer of drug-resistant trait by delivering miR-365[J]. Experimental Cell Research, 2017, 362(2):386-393.