細胞檢測
  • FA101-01.JPG
FA101-01.JPG

TransDetect? Annexin V-FITC/PI Cell Apoptosis Detection Kit


產品介紹

Annexin V 是檢測早期細胞凋亡的最靈敏指標之一。正常細胞中,磷脂酰絲氨酸 (PS)只分布在細胞膜磷脂雙分子層的內側,而在細胞凋亡早期,細胞質膜內側的磷脂酰絲氨酸發生外翻,暴露于細胞質膜的外側。Annexin V一種與磷脂酰絲氨酸有高度親和力的Ca2+依賴性磷脂結合蛋白,因此能夠利用Annexin V與細胞外側暴露的磷脂酰絲氨酸相結合的性質,檢測細胞的早期凋亡。將 Annexin V進行綠色熒光 (FITC) 標記,以標記了的Annexin V-FITC 作為探針,利用流式細胞儀或熒光顯微鏡可檢測細胞凋亡的發生。碘化丙啶 (PI)是一種核酸染料,它不能透過具備完整細胞膜的正常細胞和早期凋亡細胞,但能夠透過凋亡中晚期的細胞和壞死細胞的細胞膜而將細胞核染色,在特定激發光條件下呈現紅色。

 Annexin V-FITC PI 匹配使用,就可以將處于不同凋亡時期的細胞區分開來。在雙變量流式細胞儀的散點圖上,若橫坐標表示Annexin V信號,縱坐標表示PI信號,則左下象限 (Annexin V-/PI-) 代表活細胞;右下象限(Annexin V+ /PI-) 代表早期凋亡細胞;右上象限(Annexin V+ /PI+)代表凋亡晚期細胞和壞死細胞;左上象限(Annexin V-/PI+) 被認為是許可范圍內的檢測誤差。


References

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